Effects of Sacha Inchi (Plukenetia volubilis) Oil on Serum Interleukin 6 Levels and Foam Cell Count in Male Wistar Rats (Rattus norvegicus) Fed a High Fat and Fructose Diet
DOI:
https://doi.org/10.46799/ajesh.v3i5.313Keywords:
High Fat and Fructose Diet, IL-6, Cell Count of Foam, Sacha Inchi OilAbstract
A high-fat, high-fructose diet induces pro-inflammatory agents such as Interleukin-6 (IL-6) and hyperlipidemia, leading to atherosclerosis. Sacha inchi oil, rich in alpha-linolenic acid (ALA), has anti-inflammatory and lipid-modulating properties. This study aimed to examine the effect of sacha inchi oil on serum IL-6 levels and foam cell count in the aorta of wistar rats on a high-fat, high-fructose diet. This experiment is a true experimental study with a post-test-only control group design, 30 male wistar rats were divided into five groups: normal control (K0), high fat and fructose diet (HFFD) (K1), and three treatment groups receiving HFFD with sacha inchi oil at doses of 0.27 ml/200gBW/day (P1), 0.54 ml/200gBW/day (P2), and 1.08 ml/200gBW/day (P3) for 28 days. On day 29, serum IL-6 levels were measured using ELISA. Mean serum IL-6 levels were K1 (8.465 ng/L), P1 (4.294 ng/L), P2 (3.197 ng/L), P3 (1.379 ng/L), and K0 (1.340 ng/L), with significant differences among groups (p<0.05) except between K0 and P3 (p>0.05). Mean foam cell counts were K1 (55.3 cells/fov), P1 (47.1 cells/fov), P2 (53.2 cells/fov), P3 (52.6 cells/fov), and K0 (44.4 cells/fov), with significant differences between K0 and K1, K1 and P1, K0 and P2, and K0 and P3 (p<0.05). Sacha inchi oil significantly reduced IL-6 levels at all doses compared to the negative control (p<0.05), with the 1.08 ml/200 g/day dose being most effective. The 0.27 ml/200 g/day dose inhibited foam cell formation in HFFD rat. In conclusion, sacha inchi oil reduces serum IL-6 levels and inhibits foam cell formation in male wistar rats on a high-fat, high-fructose diet.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Debby Chesilya Wijaya, Ni Made Linawati, I Made Pande Dwipayana, Ni Wayan Winarti, Wayan Sugiritama, Ida Bagus Nyoman Putra Dwija
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International. that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
